Application
RAB10 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB10 antibody detected the RAB10 protein (arrow).Application
RAB10 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAB10 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB10; Ras-related protein Rab-10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAB10 Ras-related protein Rab-10 |
|---|---|
| Molecular Weight(MW) | 22541 Da |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion |
| Tissue Specificity | This RAB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human RAB10. |
| Cellular Localization | Cytoplasmic vesicle membrane; Lipid-anchor (Probable); Cytoplasmic side (Probable). Golgi apparatus, trans- Golgi network membrane (By similarity). Endosome membrane Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane (By similarity). Cell projection, cilium. Endoplasmic reticulum membrane. Note=Associates with SLC2A4/GLUT4 storage vesicles. Localizes to the base of the cilium. Transiently associates with phagosomes (By similarity). According to PubMed:23263280 localizes to the endoplasmic reticulum at domains of new tubule growth |
| Entrez Gene | 10890 |
|---|---|
| Nucleotide Accession | NP_057215.3 |
Application
RAB10 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB10 antibody detected the RAB10 protein (arrow).
Application
RAB10 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
