Application
Western blot analysis of RAB23 (arrow) using rabbit polyclonal RAB23 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAB23 gene (Lane 2) (Origene Technologies).| Product Name | RAB23 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB23; Ras-related protein Rab-23 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAB23 Ras-related protein Rab-23 |
|---|---|
| Molecular Weight(MW) | 26659 Da |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes |
| Tissue Specificity | This RAB23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208~237 amino acids from the C-terminal region of human RAB23. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Cell membrane. Cytoplasm. Cytoplasmic vesicle, autophagosome. Endosome membrane (By similarity) Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side (By similarity) Note=Recruited to phagosomes containing S.aureus or M.tuberculosis |
| Entrez Gene | 51715 |
|---|
Application
Western blot analysis of RAB23 (arrow) using rabbit polyclonal RAB23 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAB23 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
