Application
Fluorescent image of U251 cells stained with RAB24 (Y17) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1819e RAB24 (Y17) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). RAB24 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Western blot analysis of RAB24 (arrow) using rabbit polyclonal RAB24 Antibody (Y17). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAB24 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with RAB24 Antibody (Y17) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | RAB24 Antibody (Y17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB24; Ras-related protein Rab-24 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAB24 Ras-related protein Rab-24 |
|---|---|
| Molecular Weight(MW) | 23124 Da |
| Function | May be involved in autophagy-related processes (By similarity) |
| Tissue Specificity | This RAB24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids surrounding Y17 of human RAB24. |
| Cellular Localization | Cytoplasm, cytosol. Membrane; Lipid-anchor (By similarity). Note=Only about 20-25% is recovered in the particulate fraction |
| Entrez Gene | 53917 |
|---|
Application
Fluorescent image of U251 cells stained with RAB24 (Y17) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1819e RAB24 (Y17) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). RAB24 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Western blot analysis of RAB24 (arrow) using rabbit polyclonal RAB24 Antibody (Y17). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAB24 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with RAB24 Antibody (Y17) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
