Application
Western blot analysis of RAB3IP Antibody (Center) in HL-60 cell line lysates (35ug/lane). RAB3IP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB3IP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | RAB3IP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB3IP; RABIN8; Rab-3A-interacting protein; Rabin-3; SSX2-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RABIN8 |
|---|---|
| Alternative Names | RAB3IP RABIN8 Rab-3A-interacting protein Rabin-3 SSX2-interacting protein |
| Molecular Weight(MW) | 53021 Da |
| Function | Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP- bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis |
| Tissue Specificity | Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin |
| Entrez Gene | 117177 |
|---|
Application
Western blot analysis of RAB3IP Antibody (Center) in HL-60 cell line lysates (35ug/lane). RAB3IP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB3IP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
