Application
Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.Application
Rab5 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Rab5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB5A; RAB5; Ras-related protein Rab-5A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAB5 |
|---|---|
| Alternative Names | RAB5A RAB5 Ras-related protein Rab-5A |
| Molecular Weight(MW) | 23659 Da |
| Function | Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension |
| Tissue Specificity | This Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186~215 amino acids from the C-terminal region of human Rab5. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Early endosome membrane; Lipid-anchor (By similarity). Melanosome. Cytoplasmic vesicle (By similarity). Cell projection, ruffle (By similarity). Note=Enriched in stage I melanosomes |
| Entrez Gene | 5868 |
|---|
Application
Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Application
Rab5 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
