Application
A. Western blot analysis on A431 cell lysates using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a), 1:1000 dilution. Cells were either (A) untreated (B) treated with Lambda PhosphataseApplication
B. Immunohistochemical analysis of A431-2 using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a).| Product Name | Rac-1 Antibody Phospho (pS71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC42, Cell division control protein 42 homolog, G25K GTP-binding protein |
| Modification | p-S71 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP221Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC42 Cell division control protein 42 homolog G25K GTP-binding protein |
|---|---|
| Molecular Weight(MW) | 21259 Da |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration |
| Tissue Specificity | A phospho-specific peptide corresponding to residues surrounding Serine 71 of human Rac1/Cdc42 was used as an immunogen. The antibody only detects Rac1/Cdc42 phosphorylated on Serine 71. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Note=Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome |
| Entrez Gene | 998 |
|---|
Application
A. Western blot analysis on A431 cell lysates using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a), 1:1000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase
Application
B. Immunohistochemical analysis of A431-2 using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a).| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
