Application
RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).Application
RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term)(Cat#AP11461a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RACGAP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RACGAP1; KIAA1478; MGCRACGAP; Rac GTPase-activating protein 1; Male germ cell RacGap; Protein CYK4 homolg |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1478 MGCRACGAP |
|---|---|
| Alternative Names | RACGAP1 KIAA1478 MGCRACGAP Rac GTPase-activating protein 1 Male germ cell RacGap Protein CYK4 homolg |
| Molecular Weight(MW) | 71027 Da |
| Function | Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells |
| Tissue Specificity | Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome Cleavage furrow. Midbody. Note=Colocalizes with RND2 in Golgi- derived proacrosomal vesicles and the acrosome (By similarity) During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase |
| Entrez Gene | 29127 |
|---|---|
| Nucleotide Accession | NP_001119575.1 NP_037409.2 |
Application
RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).
Application
RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term)(Cat#AP11461a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
