Application
Western blot analysis of RAD21 antibody (C-term) (Cat.# AP7396b) in Jurkat cell line lysates (35ug/lane). RAD21 (arrow) was detected using the purified Pab.Application
RAD21 Antibody (C-term) (RB18775) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAD21 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RAD21 Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAD21 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; Nuclear matrix protein 1; SCC1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HR21 KIAA0078 NXP1 |
|---|---|
| Alternative Names | RAD21 HR21 KIAA0078 NXP1 Double-strand-break repair protein rad21 homolog Nuclear matrix protein 1 SCC1 homolog |
| Molecular Weight(MW) | 71690 Da |
| Function | Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway |
| Tissue Specificity | This RAD21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539~568 amino acids from the C-terminal region of human RAD21. |
| Cellular Localization | Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis |
| Entrez Gene | 5885 |
|---|
Application
Western blot analysis of RAD21 antibody (C-term) (Cat.# AP7396b) in Jurkat cell line lysates (35ug/lane). RAD21 (arrow) was detected using the purified Pab.
Application
RAD21 Antibody (C-term) (RB18775) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAD21 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RAD21 Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
