Application
Western blot analysis of RAD51 Antibody (Center) in 293 cell line lysates (35ug/lane). RAD51 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with RAD51 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
RAD51 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of RAD51 Antibody (Center)(Cat#AP2825c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | RAD51 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; RAD51 homolog A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAD51A RECA |
|---|---|
| Alternative Names | RAD51 RAD51A RECA DNA repair protein RAD51 homolog 1 RAD51 homolog A |
| Molecular Weight(MW) | 36966 Da |
| Function | Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 |
| Tissue Specificity | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary Weakly expressed in breast |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels |
| Entrez Gene | 5888 |
|---|
Application
Western blot analysis of RAD51 Antibody (Center) in 293 cell line lysates (35ug/lane). RAD51 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with RAD51 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
RAD51 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of RAD51 Antibody (Center)(Cat#AP2825c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
