Application
Rad9 Antibody (pS387) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Rad9 antibody detected the Rad9 protein (arrow).Application
Rad9 Antibody (S387) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Rad9 Antibody (S387)(Cat#AP11518a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Rad9 Antibody (S387) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD9A; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAD9A Cell cycle checkpoint control protein RAD9A DNA repair exonuclease rad9 homolog A |
|---|---|
| Molecular Weight(MW) | 42547 Da |
| Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex |
| Tissue Specificity | This RAD9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human RAD9A. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5883 |
|---|---|
| Nucleotide Accession | NP_004575.1 |
Application
Rad9 Antibody (pS387) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Rad9 antibody detected the Rad9 protein (arrow).
Application
Rad9 Antibody (S387) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Rad9 Antibody (S387)(Cat#AP11518a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
