Application
Western blot analysis of hRai3-W343 in T47D cell line lysates (35ug/lane). RAI3 (arrow) was detected using the purified Pab.| Product Name | RAI3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPRC5A; GPCR5A; RAI3; RAIG1; Retinoic acid-induced protein 3; G-protein coupled receptor family C group 5 member A; Orphan G-protein-coupling receptor PEIG-1; Retinoic acid-induced gene 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPCR5A RAI3 RAIG1 |
|---|---|
| Alternative Names | GPRC5A GPCR5A RAI3 RAIG1 Retinoic acid-induced protein 3 G-protein coupled receptor family C group 5 member A Orphan G-protein-coupling receptor PEIG-1 Retinoic acid-induced gene 1 protein |
| Molecular Weight(MW) | 40251 Da |
| Function | Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways |
| Tissue Specificity | Expressed at high level in fetal and adult lung tissues. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein Note=Localized in the plasma membrane and perinuclear vesicles |
Application
Western blot analysis of hRai3-W343 in T47D cell line lysates (35ug/lane). RAI3 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
