Application
RAG2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RAG2 antibody detected the RAG2 protein (arrow).Application
RAG2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the RAG2 antibody detected the RAG2 protein (arrow).Application
RAG2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAG2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RAG2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAG2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAG2; V(D)J recombination-activating protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RAG2 V(D)J recombination-activating protein 2 |
|---|---|
| Molecular Weight(MW) | 59241 Da |
| Function | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B- cell antigen receptor (BCR) expressed on individual B-lymphocytes The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity) |
| Tissue Specificity | Cells of the B- and T-lymphocyte lineages. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 5897 |
|---|---|
| Nucleotide Accession | NP_000527.2 |
Application
RAG2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RAG2 antibody detected the RAG2 protein (arrow).
Application
RAG2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the RAG2 antibody detected the RAG2 protein (arrow).
Application
RAG2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAG2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RAG2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
