Application
RANBP5 Antibody (C-term) western blot analysis in A2058 cell line lysates (15ug/lane).This demonstrates the RANBP5 antibody detected the RANBP5 protein (arrow).Application
RANBP5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RANBP5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RANBP5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IPO5; KPNB3; RANBP5; Importin-5; Importin subunit beta-3; Karyopherin beta-3; Ran-binding protein 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KPNB3 RANBP5 |
|---|---|
| Alternative Names | IPO5 KPNB3 RANBP5 Importin-5 Importin subunit beta-3 Karyopherin beta-3 Ran-binding protein 5 |
| Molecular Weight(MW) | 123630 Da |
| Function | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev |
| Tissue Specificity | This RANBP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-823 amino acids from the C-terminal region of human RANBP5. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus |
Application
RANBP5 Antibody (C-term) western blot analysis in A2058 cell line lysates (15ug/lane).This demonstrates the RANBP5 antibody detected the RANBP5 protein (arrow).
Application
RANBP5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RANBP5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
