Application
Western blot analysis of RAPGEF4 Antibody (N-term) in 293 cell line lysates (35ug/lane). RAPGEF4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAPGEF4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of 293 cells using RAPGEF4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAPGEF4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAPGEF4; CGEF2; EPAC2; Rap guanine nucleotide exchange factor 4; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; cAMP-regulated guanine nucleotide exchange factor II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CGEF2 EPAC2 |
|---|---|
| Alternative Names | RAPGEF4 CGEF2 EPAC2 Rap guanine nucleotide exchange factor 4 Exchange factor directly activated by cAMP 2 Exchange protein directly activated by cAMP 2 cAMP-regulated guanine nucleotide exchange factor II |
| Molecular Weight(MW) | 115522 Da |
| Function | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2 (By similarity) |
| Tissue Specificity | Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein (By similarity) |
| Entrez Gene | 11069 |
|---|
Application
Western blot analysis of RAPGEF4 Antibody (N-term) in 293 cell line lysates (35ug/lane). RAPGEF4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAPGEF4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of 293 cells using RAPGEF4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
