Application
RARB Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).Application
Fluorescent confocal image of Hela cell stained with RARB Antibody (Center)(Cat#AP18153c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.| Product Name | RARB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RARB; HAP; NR1B2; Retinoic acid receptor beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HAP NR1B2 |
|---|---|
| Alternative Names | RARB HAP NR1B2 Retinoic acid receptor beta HBV-activated protein Nuclear receptor subfamily 1 group B member 2 RAR-epsilon |
| Molecular Weight(MW) | 50489 Da |
| Function | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function |
| Tissue Specificity | This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-195 amino acids from the Central region of human RARB. |
| Cellular Localization | Isoform Beta-1: Nucleus. Isoform Beta-4: Cytoplasm. |
| Entrez Gene | 5915 |
|---|---|
| Nucleotide Accession | NP_000956.2 |
Application
RARB Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with RARB Antibody (Center)(Cat#AP18153c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
