Application
RARS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).Application
RARS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of RARS Antibody (C-term)(Cat#AP11091b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
RARS Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RARS Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RARS; Arginine--tRNA ligase, cytoplasmic; Arginyl-tRNA synthetase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RARS Arginine--tRNA ligase cytoplasmic Arginyl-tRNA synthetase |
|---|---|
| Molecular Weight(MW) | 75379 Da |
| Function | Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1 |
| Tissue Specificity | This RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 612-642 amino acids from the C-terminal region of human RARS. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 5917 |
|---|---|
| Nucleotide Accession | NP_002878.2 |
Application
RARS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).
Application
RARS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of RARS Antibody (C-term)(Cat#AP11091b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
RARS Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
