Application
RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).Application
RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RASGRP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RASGRP |
|---|---|
| Alternative Names | RASGRP1 RASGRP RAS guanyl-releasing protein 1 Calcium and DAG-regulated guanine nucleotide exchange factor II Ras guanyl-releasing protein |
| Molecular Weight(MW) | 90402 Da |
| Function | Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation |
| Tissue Specificity | Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level) |
| Cellular Localization | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells (By similarity) |
| Entrez Gene | 10125 |
|---|---|
| Nucleotide Accession | NP_005730.2 NP_001122074.1 |
Application
RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).
Application
RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
