Application
Western blot analysis of RAT MIF Antibody (C-term) in A375 cell line lysates (35ug/lane). MIF (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with RAT MIF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | RAT MIF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mif; Macrophage migration inhibitory factor; Glutathione-binding 13 kDa protein; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Mif Macrophage migration inhibitory factor Glutathione-binding 13 kDa protein L-dopachrome isomerase L-dopachrome tautomerase Phenylpyruvate tautomerase |
|---|---|
| Molecular Weight(MW) | 12477 Da |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity) |
| Tissue Specificity | Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney |
| Cellular Localization | Secreted (By similarity). Cytoplasm (By similarity). Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens (By similarity) |
| Entrez Gene | 81683 |
|---|
Application
Western blot analysis of RAT MIF Antibody (C-term) in A375 cell line lysates (35ug/lane). MIF (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with RAT MIF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
