Application
Dot blot analysis of rat TSC1 Antibody (Phospho S312) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Product Name | rat TSC1(Phospho S312) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tsc1; Hamartin; Tuberous sclerosis 1 protein homolog |
| Modification | p-S312 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Tsc1 Hamartin Tuberous sclerosis 1 protein homolog |
|---|---|
| Molecular Weight(MW) | 129022 Da |
| Function | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity) |
| Tissue Specificity | Highly expressed in brain, spleen and kidney, followed by liver and heart |
| Cellular Localization | Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). Note=At steady state found in association with membranes (By similarity) |
| Entrez Gene | 60445 |
|---|---|
| Nucleotide Accession | NP_068626.1 |
Application
Dot blot analysis of rat TSC1 Antibody (Phospho S312) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
