Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-p130 (pS952) RabMAb (Cat. #AJ1683c), 1:500000 dilution. Cells were either (A) untreated (B) treated with AP.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-phospho p130 RabMAb (Cat. #AJ1683c).| Product Name | Rb2 (p130) Antibody Phospho (pS952) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBL2, RB2, Retinoblastoma-like protein 2, PRB2;130 kDa retinoblastoma-associated protein;p130 |
| Modification | p-S952 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2387AY |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RB2 |
|---|---|
| Alternative Names | RBL2 RB2 Retinoblastoma-like protein 2 PRB2 130 kDa retinoblastoma-associated protein p130 |
| Molecular Weight(MW) | 128367 Da |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor |
| Tissue Specificity | A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5934 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-p130 (pS952) RabMAb (Cat. #AJ1683c), 1:500000 dilution. Cells were either (A) untreated (B) treated with AP.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-phospho p130 RabMAb (Cat. #AJ1683c).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
