Application
Western blot analysis of RBL2/p130 antibody (N-term) (Cat.# AP6543a) in NIH-3T3 cell line lysates (35ug/lane). RBL2 (arrow) was detected using the purified Pab.Application
RBL2/p130 Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RBL2/p130 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBL2; RB2; Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; Retinoblastoma-related protein 2; pRb2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RB2 |
|---|---|
| Alternative Names | RBL2 RB2 Retinoblastoma-like protein 2 130 kDa retinoblastoma-associated protein Retinoblastoma-related protein 2 pRb2 |
| Molecular Weight(MW) | 128367 Da |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor |
| Tissue Specificity | This RBL2/p130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157~187 amino acids from the N-terminal region of human RBL2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5934 |
|---|
Application
Western blot analysis of RBL2/p130 antibody (N-term) (Cat.# AP6543a) in NIH-3T3 cell line lysates (35ug/lane). RBL2 (arrow) was detected using the purified Pab.
Application
RBL2/p130 Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
