Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Rb (pT821) RabMAb (Cat. #AJ1682e); dilution 1:1,000. Cells were (1) untreated, and (2) treated with Phosphatase.Application
B. Immunofluorescent analysis of HeLa cells using anti-Phospho-Rb (pT821) RabMAb (Cat. #AJ1682e).| Product Name | Rb Antibody Phospho (pT821) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RB1, Retinoblastoma-associated protein, pp110;p105-Rb |
| Modification | p-T821 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E231 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RB1 Retinoblastoma-associated protein pp110 p105-Rb |
|---|---|
| Molecular Weight(MW) | 106159 Da |
| Function | Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1 Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity |
| Tissue Specificity | Expressed in the retina. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5925 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Rb (pT821) RabMAb (Cat. #AJ1682e); dilution 1:1,000. Cells were (1) untreated, and (2) treated with Phosphatase.
Application
B. Immunofluorescent analysis of HeLa cells using anti-Phospho-Rb (pT821) RabMAb (Cat. #AJ1682e).| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
