Application
RBM22 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM22 antibody detected the RBM22 protein (arrow).Application
Western blot analysis of RBM22 (arrow) using rabbit polyclonal RBM22 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM22 gene.Application
RBM22 Antibody (Center) (AP14906c)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM22 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RBM22 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZC3H16 |
|---|---|
| Alternative Names | RBM22 ZC3H16 Pre-mRNA-splicing factor RBM22 RNA-binding motif protein 22 Zinc finger CCCH domain-containing protein 16 |
| Molecular Weight(MW) | 46896 Da |
| Function | Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses |
| Tissue Specificity | This RBM22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the Central region of human RBM22. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol |
| Entrez Gene | 55696 |
|---|---|
| Nucleotide Accession | NP_060517.1 |
Application
RBM22 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM22 antibody detected the RBM22 protein (arrow).
Application
Western blot analysis of RBM22 (arrow) using rabbit polyclonal RBM22 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM22 gene.
Application
RBM22 Antibody (Center) (AP14906c)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM22 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
