Application
RC3H1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RC3H1 antibody detected the RC3H1 protein (arrow).Application
RC3H1 Antibody (C-term) (AP14162b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RC3H1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RC3H1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RC3H1; KIAA2025; RNF198; Roquin; RING finger and C3H zinc finger protein 1; RING finger protein 198 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA2025 RNF198 |
|---|---|
| Alternative Names | RC3H1 KIAA2025 RNF198 Roquin RING finger and C3H zinc finger protein 1 RING finger protein 198 |
| Molecular Weight(MW) | 125736 Da |
| Function | Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1 (By similarity) |
| Tissue Specificity | Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver |
| Cellular Localization | Cytoplasm (By similarity). Note=Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA (By similarity) |
| Entrez Gene | 149041 |
|---|---|
| Nucleotide Accession | NP_742068.1 |
Application
RC3H1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RC3H1 antibody detected the RC3H1 protein (arrow).
Application
RC3H1 Antibody (C-term) (AP14162b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RC3H1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
