Application
Western blot analysis of anti-RCHY1 Antibody (N-term H44) in HL60 cell line lysates (35ug/lane). RCHY1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with STK32C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
RCHY1 Antibody (N-term H44) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RCHY1 Antibody (N-term H44) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RCHY1; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; RING finger and CHY zinc finger domain-containing protein 1; Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; Zin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARNIP CHIMP PIRH2 RNF199 ZNF363 |
|---|---|
| Alternative Names | RCHY1 ARNIP CHIMP PIRH2 RNF199 ZNF363 RING finger and CHY zinc finger domain-containing protein 1 Androgen receptor N-terminal-interacting protein CH-rich-interacting match with PLAG1 E3 ubiquitin-protein ligase Pirh2 RING finger protein 199 Zin |
| Molecular Weight(MW) | 30110 Da |
| Function | Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53 Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity |
| Tissue Specificity | This RCHY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~66 amino acids from the N-terminal region of human RCHY1. |
| Cellular Localization | Nucleus. Nucleus speckle. Cytoplasm. |
| Entrez Gene | 25898 |
|---|
Application
Western blot analysis of anti-RCHY1 Antibody (N-term H44) in HL60 cell line lysates (35ug/lane). RCHY1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with STK32C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
RCHY1 Antibody (N-term H44) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
