Application
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.Application
Figure 2: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).| Product Name | Pirh2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARNIP; CHIMP; RNF199; RCHY1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H10 |
| Host Species | Mouse |
| Tested Applications | WBIFFC |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARNIP CHIMP PIRH2 RNF199 ZNF363 |
|---|---|
| Alternative Names | ARNIP CHIMP RNF199 RCHY1 |
| Molecular Weight(MW) | 30110 Da |
| Function | Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53 Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity |
| Tissue Specificity | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
| Cellular Localization | Nucleus. Nucleus speckle. Cytoplasm. |
| Entrez Gene | 25898 |
|---|---|
| Nucleotide Accession | NP_056251.2 |
Application
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Application
Figure 2: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
