Application
Western blot analysis of RUVBL2(arrow) using rabbit polyclonal RUVBL2 antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RUVBL2 gene (Lane 2) (Origene Technologies).| Product Name | Reptin/TIP49B/RUVB2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUVBL2; INO80J; TIP48; TIP49B; RuvB-like 2; 48 kDa TATA box-binding protein-interacting protein; 51 kDa erythrocyte cytosolic protein; INO80 complex subunit J; Repressing pontin 52; TIP49b; TIP60-associated protein 54-beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INO80J TIP48 TIP49B |
|---|---|
| Alternative Names | RUVBL2 INO80J TIP48 TIP49B RuvB-like 2 48 kDa TATA box-binding protein-interacting protein 51 kDa erythrocyte cytosolic protein INO80 complex subunit J Repressing pontin 52 TIP49b TIP60-associated protein 54-beta |
| Molecular Weight(MW) | 51157 Da |
| Function | Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair |
| Tissue Specificity | Ubiquitously expressed. Highly expressed in testis and thymus |
| Cellular Localization | Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes |
Application
Western blot analysis of RUVBL2(arrow) using rabbit polyclonal RUVBL2 antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RUVBL2 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
