Application
Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).Application
Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.Application
RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RFC5 Antibody (Center R185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RFC5; Replication factor C subunit 5; Activator 1 36 kDa subunit; Activator 1 subunit 5; Replication factor C 36 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RFC5 Replication factor C subunit 5 Activator 1 36 kDa subunit Activator 1 subunit 5 Replication factor C 36 kDa subunit |
|---|---|
| Molecular Weight(MW) | 38497 Da |
| Function | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1 |
| Tissue Specificity | This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the Center region of human RFC5. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 5985 |
|---|
Application
Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).
Application
Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
Application
RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
