Application
RFWD2 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RFWD2 antibody detected the RFWD2 protein (arrow).| Product Name | RFWD2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RFWD2; COP1; RNF200; E3 ubiquitin-protein ligase RFWD2; Constitutive photomorphogenesis protein 1 homolog; RING finger and WD repeat domain protein 2; RING finger protein 200 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COP1 RNF200 |
|---|---|
| Alternative Names | RFWD2 COP1 RNF200 E3 ubiquitin-protein ligase RFWD2 Constitutive photomorphogenesis protein 1 homolog RING finger and WD repeat domain protein 2 RING finger protein 200 |
| Molecular Weight(MW) | 80474 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1 Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival |
| Tissue Specificity | Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart |
| Cellular Localization | Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles |
| Entrez Gene | 64326 |
|---|---|
| Nucleotide Accession | NP_071902.2 |
Application
RFWD2 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RFWD2 antibody detected the RFWD2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
