Application
RGMA Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RGMA antibody detected the RGMA protein (arrow).| Product Name | RGMA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RGM |
|---|---|
| Alternative Names | RGMA RGM Repulsive guidance molecule A RGM domain family member A |
| Molecular Weight(MW) | 49347 Da |
| Function | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8 |
| Tissue Specificity | This RGMA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the C-terminal region of human RGMA. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor (By similarity) |
| Entrez Gene | 56963 |
|---|---|
| Nucleotide Accession | NP_001159758.1 |
Application
RGMA Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RGMA antibody detected the RGMA protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
