Application
RHOH Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RHOH antibody detected the RHOH protein (arrow).| Product Name | RHOH Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHOH; ARHH; TTF; Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARHH TTF |
|---|---|
| Alternative Names | RHOH ARHH TTF Rho-related GTP-binding protein RhoH GTP-binding protein TTF Translocation three four protein |
| Molecular Weight(MW) | 21331 Da |
| Function | Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42 Negatively regulates leukotriene production in neutrophils |
| Tissue Specificity | Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis |
| Cellular Localization | Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Note=Co-localizes together with ZAP70 in the immunological synapse (By similarity) |
| Entrez Gene | 399 |
|---|---|
| Nucleotide Accession | NP_004301.1 |
Application
RHOH Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RHOH antibody detected the RHOH protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
