Application
A. Western blot analysis on (A) HL-60 + Retinoic acid (B) HL-60 cell lysates and (C) Human thymus lysate using anti-RIAM RabMAb (Cat. #AJ1689a), dilution 1:2000.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-RIAM RabMAb (Cat. #AJ1689a).| Product Name | RIAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2805Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PREL1, RARP1, RIAM |
|---|---|
| Alternative Names | APBB1IP PREL1 RARP1 RIAM Amyloid beta A4 precursor protein-binding family B member 1-interacting protein Proline-rich protein 73 Retinoic acid-responsive proline-rich protein 1 |
| Molecular Weight(MW) | 73183 Da |
| Function | Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion |
| Tissue Specificity | Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity). Cell projection, lamellipodium (By similarity) Cell junction, focal adhesion (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity) |
| Entrez Gene | 54518 |
|---|
Application
A. Western blot analysis on (A) HL-60 + Retinoic acid (B) HL-60 cell lysates and (C) Human thymus lysate using anti-RIAM RabMAb (Cat. #AJ1689a), dilution 1:2000.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-RIAM RabMAb (Cat. #AJ1689a).| Application Notes | WB:1:1000~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
