Application
The anti-RPN1 Pab is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).| Product Name | Ribophorin (RPN1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPN1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; Ribophorin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RPN1 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit Ribophorin I Ribophorin-1 |
|---|---|
| Molecular Weight(MW) | 68569 Da |
| Function | Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains |
| Tissue Specificity | Expressed in all tissues tested. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 6184 |
|---|
Application
The anti-RPN1 Pab is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
