Application
RICTOR Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the RICTOR antibody detected the RICTOR protein (arrow).Application
RICTOR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RICTOR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RICTOR Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RICTOR; KIAA1999; Rapamycin-insensitive companion of mTOR; AVO3 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1999 |
|---|---|
| Alternative Names | RICTOR KIAA1999 Rapamycin-insensitive companion of mTOR AVO3 homolog |
| Molecular Weight(MW) | 192218 Da |
| Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development |
| Tissue Specificity | This RICTOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1627~1656 amino acids from the C-terminal region of human RICTOR. |
| Entrez Gene | 253260 |
|---|
Application
RICTOR Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the RICTOR antibody detected the RICTOR protein (arrow).
Application
RICTOR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RICTOR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
