Application
Western blot analysis of Rictor Antibody (N-term) in Ramos cell line lysates (35ug/lane). RICTOR (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Rictor Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RICTOR; KIAA1999; Rapamycin-insensitive companion of mTOR; AVO3 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1999 |
|---|---|
| Alternative Names | RICTOR KIAA1999 Rapamycin-insensitive companion of mTOR AVO3 homolog |
| Molecular Weight(MW) | 192218 Da |
| Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development |
| Tissue Specificity | This Rictor antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the N-terminal region of human Rictor. |
| Entrez Gene | 253260 |
|---|
Application
Western blot analysis of Rictor Antibody (N-term) in Ramos cell line lysates (35ug/lane). RICTOR (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
