Application
Western blot analysis of anti-RIPK2 Pab in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Western blot analysis of anti-RIPK2 Pab in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.Application
Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.| Product Name | RIPK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CARDIAK, RICK, RIP2 |
|---|---|
| Alternative Names | RIPK2 CARDIAK RICK RIP2 Receptor-interacting serine/threonine-protein kinase 2 CARD-containing interleukin-1 beta-converting enzyme-associated kinase RIP-like-interacting CLARP kinase Receptor-interacting protein 2 Tyrosine-protein kinase RIPK2 |
| Molecular Weight(MW) | 61195 Da |
| Function | Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation |
| Tissue Specificity | Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node |
| Cellular Localization | Cytoplasm (Probable). |
Application
Western blot analysis of anti-RIPK2 Pab in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Western blot analysis of anti-RIPK2 Pab in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
