Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-RKIP (pS153) RabMAb (Cat. #AJ1691a), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.Application
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-RKIP RabMAb (Cat. #AJ1691a).| Product Name | RKIP Antibody Phospho (pS153) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PEBP1, PBP, PEBP, Hippocampal cholinergic neurostimulating peptide, Prostatic-binding protein;HCNPpp;Neuropolypeptide h3;Raf kinase inhibitor protein |
| Modification | p-S153 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2845Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PBP PEBP |
|---|---|
| Alternative Names | PEBP1 PBP PEBP Hippocampal cholinergic neurostimulating peptide Prostatic-binding protein HCNPpp Neuropolypeptide h3 Raf kinase inhibitor protein |
| Molecular Weight(MW) | 21057 Da |
| Function | Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation |
| Tissue Specificity | A phospho-specific peptide corresponding to residues surrounding serine 153 of human RKIP was used as an immunogen. This antibody detects RKIP phosphorylated on serine 153. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 5037 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-RKIP (pS153) RabMAb (Cat. #AJ1691a), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
Application
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-RKIP RabMAb (Cat. #AJ1691a).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
