Application
RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).Application
RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).| Product Name | RN185 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF185; E3 ubiquitin-protein ligase RNF185; RING finger protein 185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RNF185 E3 ubiquitin-protein ligase RNF185 RING finger protein 185 |
|---|---|
| Molecular Weight(MW) | 20459 Da |
| Function | Mitochondrial E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 |
| Tissue Specificity | This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the Central region of human RN185. |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein |
| Entrez Gene | 91445 |
|---|---|
| Nucleotide Accession | NP_689480.2 |
Application
RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
Application
RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
