Application
RNASE3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).Application
Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#AP13557b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | RNASE3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNASE3; ECP; RNS3; Eosinophil cationic protein; Ribonuclease 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ECP RNS3 |
|---|---|
| Alternative Names | RNASE3 ECP RNS3 Eosinophil cationic protein Ribonuclease 3 |
| Molecular Weight(MW) | 18385 Da |
| Function | Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content |
| Tissue Specificity | This RNASE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-151 amino acids from the C-terminal region of human RNASE3. |
| Cellular Localization | Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus |
| Entrez Gene | 6037 |
|---|---|
| Nucleotide Accession | NP_002926.2 |
Application
RNASE3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).
Application
Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#AP13557b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
