Application
RNF111 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RNF111 antibody detected the RNF111 protein (arrow).| Product Name | RNF111 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RNF111 E3 ubiquitin-protein ligase Arkadia RING finger protein 111 |
|---|---|
| Molecular Weight(MW) | 108862 Da |
| Function | Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin- protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation |
| Tissue Specificity | Broadly expressed. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Upon TGF-beta treatment, translocates from nucleus to cytosol |
| Entrez Gene | 54778 |
|---|---|
| Nucleotide Accession | NP_060080.6 |
Application
RNF111 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RNF111 antibody detected the RNF111 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
