Application
RNF144B Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RNF144B antibody detected the RNF144B protein (arrow).| Product Name | RNF144B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IBRDC2 P53RFP |
|---|---|
| Alternative Names | RNF144B IBRDC2 P53RFP E3 ubiquitin-protein ligase RNF144B IBR domain-containing protein 2 RING finger protein 144B p53-inducible RING finger protein |
| Molecular Weight(MW) | 33697 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se |
| Tissue Specificity | Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation |
| Entrez Gene | 255488 |
|---|---|
| Nucleotide Accession | NP_877434.2 |
Application
RNF144B Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RNF144B antibody detected the RNF144B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
