Application
RNF20 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF20 antibody detected the RNF20 protein (arrow).| Product Name | RNF20 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF20; BRE1A; E3 ubiquitin-protein ligase BRE1A; RING finger protein 20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRE1A |
|---|---|
| Alternative Names | RNF20 BRE1A E3 ubiquitin-protein ligase BRE1A RING finger protein 20 |
| Molecular Weight(MW) | 113662 Da |
| Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator |
| Tissue Specificity | This RNF20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-146 amino acids from the N-terminal region of human RNF20. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 56254 |
|---|---|
| Nucleotide Accession | NP_062538.5 |
Application
RNF20 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF20 antibody detected the RNF20 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
