Application
RNF19A Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF19A antibody detected the RNF19A protein (arrow).Application
RNF19A Antibody (Center) (AP14232c)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF19A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RNF19A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF19A; RNF19; E3 ubiquitin-protein ligase RNF19A; Double ring-finger protein; RING finger protein 19A; p38 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF19 |
|---|---|
| Alternative Names | RNF19A RNF19 E3 ubiquitin-protein ligase RNF19A Double ring-finger protein RING finger protein 19A p38 |
| Molecular Weight(MW) | 90696 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection |
| Tissue Specificity | Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system |
| Cellular Localization | Membrane; Multi-pass membrane protein (Potential). Cytoplasm, cytoskeleton, centrosome. Note=Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients |
| Entrez Gene | 25897 |
|---|---|
| Nucleotide Accession | NP_056250.3 NP_904355.1 |
Application
RNF19A Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF19A antibody detected the RNF19A protein (arrow).
Application
RNF19A Antibody (Center) (AP14232c)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF19A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
