Application
RNF31 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF31 antibody detected the RNF31 protein (arrow).| Product Name | RNF31 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF31; ZIBRA; E3 ubiquitin-protein ligase RNF31; HOIL-1-interacting protein; RING finger protein 31; Zinc in-between-RING-finger ubiquitin-associated domain protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZIBRA |
|---|---|
| Alternative Names | RNF31 ZIBRA E3 ubiquitin-protein ligase RNF31 HOIL-1-interacting protein RING finger protein 31 Zinc in-between-RING-finger ubiquitin-associated domain protein |
| Molecular Weight(MW) | 119652 Da |
| Function | E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex Binds polyubiquitin of different linkage types |
| Tissue Specificity | Expressed in both normal and transformed breast epithelial cell lines |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 55072 |
|---|---|
| Nucleotide Accession | NP_060469.4 |
Application
RNF31 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF31 antibody detected the RNF31 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
