Application
Western blot analysis of RNF40 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. RNF40 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | RNF40 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF40; BRE1B; KIAA0661; E3 ubiquitin-protein ligase BRE1B; 95 kDa retinoblastoma-associated protein; RING finger protein 40 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRE1B KIAA0661 |
|---|---|
| Alternative Names | RNF40 BRE1B KIAA0661 E3 ubiquitin-protein ligase BRE1B 95 kDa retinoblastoma-associated protein RING finger protein 40 |
| Molecular Weight(MW) | 113650 Da |
| Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes |
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine |
| Cellular Localization | Nucleus. |
| Entrez Gene | 9810 |
|---|---|
| Nucleotide Accession | NP_055586.1 |
Application
Western blot analysis of RNF40 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. RNF40 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
