Application
RNF6 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RNF6 antibody detected the RNF6 protein (arrow).| Product Name | RNF6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF6; SPG2; E3 ubiquitin-protein ligase RNF6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPG2 |
|---|---|
| Alternative Names | RNF6 SPG2 E3 ubiquitin-protein ligase RNF6 |
| Molecular Weight(MW) | 78091 Da |
| Function | E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator |
| Tissue Specificity | Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues |
| Cellular Localization | Nucleus. Cytoplasm. Cell projection, axon (By similarity). Nucleus, PML body (By similarity). Note=Localizes to the PML nuclear bodies in Sertoli cells (By similarity) |
| Entrez Gene | 6049 |
|---|---|
| Nucleotide Accession | NP_898865.1 |
Application
RNF6 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RNF6 antibody detected the RNF6 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
