Application
Western blot analysis of RNGTT antibody (C-term) in 293 cell line lysates (35ug/lane). RNGTT (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using RNGTT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RNGTT Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNGTT; CAP1A; mRNA-capping enzyme; HCAP1; HCE; Polynucleotide 5'-triphosphatase; mRNA 5'-triphosphatase; mRNA guanylyltransferase; GTP--RNA guanylyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP1A |
|---|---|
| Alternative Names | RNGTT CAP1A mRNA-capping enzyme HCAP1 HCE Polynucleotide 5'-triphosphatase mRNA 5'-triphosphatase mRNA guanylyltransferase GTP--RNA guanylyltransferase |
| Molecular Weight(MW) | 68557 Da |
| Function | Bifunctional mRNA-capping enzyme exhibiting RNA 5'- triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus |
| Tissue Specificity | Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 8732 |
|---|
Application
Western blot analysis of RNGTT antibody (C-term) in 293 cell line lysates (35ug/lane). RNGTT (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using RNGTT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
