Application
ROBLD3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ROBLD3 antibody detected the ROBLD3 protein (arrow).Application
ROBLD3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROBLD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ROBLD3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMTOR2; MAPBPIP; ROBLD3; Ragulator complex protein LAMTOR2; Endosomal adaptor protein p14; Late endosomal/lysosomal Mp1-interacting protein; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Mitogen-activated protein-binding protein-interact |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPBPIP ROBLD3 |
|---|---|
| Alternative Names | LAMTOR2 MAPBPIP ROBLD3 Ragulator complex protein LAMTOR2 Endosomal adaptor protein p14 Late endosomal/lysosomal Mp1-interacting protein Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2 Mitogen-activated protein-binding protein-interact |
| Molecular Weight(MW) | 13508 Da |
| Function | As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2 |
| Tissue Specificity | This ROBLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the Central region of human ROBLD3. |
| Cellular Localization | Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Lysosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) |
| Entrez Gene | 28956 |
|---|---|
| Nucleotide Accession | NP_054736.1 |
Application
ROBLD3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ROBLD3 antibody detected the ROBLD3 protein (arrow).
Application
ROBLD3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROBLD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
