Application
A. Western blot analysis anti-ROCK1 RabMAb on untreated HeLa cell lysate (A) and camptothecin treated HeLa lysate (B). (Cat. #AJ1692a), dilution 1:500Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-ROCK1 RabMAb (Cat. #AJ1692a).Application
C. Immunofluorescent staining of HeLa cells using anti-ROCK1 RabMAb (Cat. #AJ1692a).| Product Name | ROCK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ROCK1, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP786Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ROCK1 Rho-associated protein kinase 1 Renal carcinoma antigen NY-REN-35 |
|---|---|
| Molecular Weight(MW) | 158175 Da |
| Function | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation |
| Tissue Specificity | Detected in blood platelets. |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes |
| Entrez Gene | 6093 |
|---|
Application
A. Western blot analysis anti-ROCK1 RabMAb on untreated HeLa cell lysate (A) and camptothecin treated HeLa lysate (B). (Cat. #AJ1692a), dilution 1:500
Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-ROCK1 RabMAb (Cat. #AJ1692a).
Application
C. Immunofluorescent staining of HeLa cells using anti-ROCK1 RabMAb (Cat. #AJ1692a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
