Application
Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane). RORA (arrow) was detected using the purified Pab.Application
Fluorescent confocal image of Hela cell stained with RORA Antibody (T216)(Cat#AP1085d).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.| Product Name | RORA Antibody (T216) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RORA; NR1F1; RZRA; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NR1F1 RZRA |
|---|---|
| Alternative Names | RORA NR1F1 RZRA Nuclear receptor ROR-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 Retinoid-related orphan receptor-alpha |
| Molecular Weight(MW) | 63036 Da |
| Function | Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3' Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives |
| Tissue Specificity | Widely expressed in a number of tissues. |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane). RORA (arrow) was detected using the purified Pab.
Application
Fluorescent confocal image of Hela cell stained with RORA Antibody (T216)(Cat#AP1085d).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
